Cell-specific ATP7A transport sustains copper-dependent tyrosinase activity in melanosomes
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چکیده
منابع مشابه
Effects of ATP7A overexpression in mice on copper transport and metabolism in lactation and gestation
Placentae and mammary epithelial cells are unusual in robustly expressing two copper "pumps", ATP7A and B, raising the question of their individual roles in these tissues in pregnancy and lactation. Confocal microscopic evidence locates ATP7A to the fetal side of syncytiotrophoblasts, suggesting a role in pumping Cu towards the fetus; and to the basolateral (blood) side of lactating mammary epi...
متن کاملMammary gland copper transport is stimulated by prolactin through alterations in Ctr1 and Atp7A localization.
Milk copper (Cu) concentration declines and directly reflects the stage of lactation. Three Cu-specific transporters (Ctr1, Atp7A, Atp7B) have been identified in the mammary gland; however, the integrated role they play in milk Cu secretion is not understood. Whereas the regulation of milk composition by the lactogenic hormone prolactin (PRL) has been documented, the specific contribution of PR...
متن کاملMammalian tyrosinase; the relationship of copper to enzymatic activity.
Copper has been reported to be an essential part of the enzyme tyrosinase prepared from various plant and insect sources (l-8). In the case of potato tyrosinase, Kubowitz (1, 2) found that the enzyme could be inhibited by reagents which combine with copper, e.g. diethyldithiocarbamate, salicylaldoxime, and carbon monoxide. He demonstrated that treatment of the enzyme with cyanide followed by di...
متن کاملImmunocytochemical localization of the Menkes copper transport protein (ATP7A) to the trans-Golgi network.
We have generated polyclonal antibodies against the amino-terminal third of the Menkes protein (ATP7A; MNK) by immunizing rabbits with a histidine-tagged MNK fusion construct containing metal-binding domains 1-4. The purified antibodies were used in Western analysis of cell lysates and in indirect immunofluorescence experiments on cultured cells. On Western blots, the antibodies recognized the ...
متن کاملA role for the ATP7A copper-transporting ATPase in macrophage bactericidal activity.
Copper is an essential micronutrient that is necessary for healthy immune function. This requirement is underscored by an increased susceptibility to bacterial infection in copper-deficient animals; however, a molecular understanding of its importance in immune defense is unknown. In this study, we investigated the effect of proinflammatory agents on copper homeostasis in RAW264.7 macrophages. ...
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ژورنال
عنوان ژورنال: Nature
سال: 2008
ISSN: 0028-0836,1476-4687
DOI: 10.1038/nature07163